Score shortly after a quick throw-in

Recruit 3 Assassins.

Complete DNA Sequence 3.

Renovate 5 buildings in the Antico district.

Discover the Shrine in the Terme di Traiano.

Find a secure place to hide and re-enter the Animus.

Attempt to access DNA Sequence 9 for the first time.

Complete DNA Sequence 10.

Spend 5000 florins on Courtesans.

Discover the Assassin's Tomb inside Santa Maria della Visitazione.

Discover the Assassin's Tomb inside the Basilica di San Marco.

Complete training and reenter the Animus.