Installed the third upgrade to a weapon.

Achieved Rank 10.

The player has either Harvested or Rescued every possible Little Sister

Acquire all upgrades for all weapons

Fully research the Little Sister

The player has entered Sander Cohen's personal quarters

Fully research the Rosie

Fully research the Nitro Splicer

The player has defeated Andrew Ryan

The player has purchased every slot in one of the Plasmid or Tonic tracks

The player has completed Sander Cohen's great masterpiece

The player has fully researched the Spider Splicer

Reach level 20 in the multiplayer mode.