The player has entered Sander Cohen's personal quarters

Purchased every slot in all four Plasmid and Tonic tracks

The player has fully upgraded three weapons

Fully research the Little Sister

The player has completed Sander Cohen's great masterpiece

Fully research the Nitro Splicer

The player has fully researched the Spider Splicer

The player has restored the forests of Arcadia

The player has successfully invented at least one item

The player has purchased every slot in one of the Plasmid or Tonic tracks

The player has fully researched the Thuggish Splicer

The player has defeated Peach Wilkins