Defeat the Goliath in the single player Campaign.

Purchased every slot in all four Plasmid and Tonic tracks

The player has either Harvested or Rescued every possible Little Sister

The player has broken Fontaine's mind control

The player has entered Sander Cohen's personal quarters

The player has defeated Andrew Ryan

The player has completed Sander Cohen's great masterpiece

The player has purchased every slot in one of the Plasmid or Tonic tracks

The player has successfully hacked a safe

The player has acquired at least one weapon upgrade

The player has taken at least one Research Photo of a Splicer

The player has purchased one slot in any Plasmid or Tonic track

The player has successfully hacked a security camera