Unlocked all trophies

Complete the game on Hard difficulty without using a Vita-Chamber.

The player has completed the game on the Hard difficulty setting

The player has defeated Atlas

Purchased every slot in all four Plasmid and Tonic tracks

The player has either Harvested or Rescued every possible Little Sister

Acquire all upgrades for all weapons

Max out all possible research

The player has taken a picture of Sander Cohen's corpse

Fully upgrade four weapons

Collect or Invent 58 Tonics in the Physical, Engineering and Combat tracks

Fully research the Little Sister