Complete the game on Hard difficulty without using a Vita-Chamber.

The player has completed the game on the Hard difficulty setting

Successfully invent at least 100 items

Max out all possible research

The player has either Harvested or Rescued every possible Little Sister

Fully upgrade five weapons

The player has fully upgraded three weapons

Fully research the Nitro Splicer

Fully research the Little Sister

Collect or Invent 58 Tonics in the Physical, Engineering and Combat tracks

The player has fully upgraded two weapons

The player has completed Sander Cohen's great masterpiece

The player has fully researched the Spider Splicer