Defeat three enemies with one explosion

Defeat 20 enemies with headshots

Defeat 20 enemies with hand-to-hand combat

The player has fully upgraded two weapons

The player has completed Sander Cohen's great masterpiece

The player has successfully invented all possible ammo types

Hit the jackpot at a slot machine

The player has purchased every slot in one of the Plasmid or Tonic tracks

Fully research the Houdini Splicer

The player has fully upgraded one weapon

Fully research the Nitro Splicer

The player has defeated Peach Wilkins

The player has successfully completed 50 hacks