Purchased every slot in all four Plasmid and Tonic tracks

The player has either Harvested or Rescued every possible Little Sister

Collect or Invent 58 Tonics in the Physical, Engineering and Combat tracks

Successfully invent at least 100 items

The player has taken a picture of Sander Cohen's corpse

Fully upgrade four weapons

Fully research the Nitro Splicer

The player has purchased every slot in one of the Plasmid or Tonic tracks

The player has fully upgraded three weapons

The player has completed Sander Cohen's great masterpiece

The player has fully upgraded two weapons

The player has successfully invented all possible ammo types

The player has restored the forests of Arcadia