Dealt with every Little Sister.

Fully upgraded one Plasmid to the Level 3 version.

Defeated the Preacher.

Killed 50 enemies using only hacked Security.

Bought one Plasmid or Tonic Slot at a Gatherer's Garden.

Spent 2000 dollars at Vending Machines.

Got through a Gather with no damage and no one getting to the Little Sister.

Maxed out one Research Track.

Researched a Splicer with the Research Camera.

Defended yourself against the Big Sister without dying.

Hacked one of each kind of machine.

Upgraded a weapon at a Power to the People Station.