Complete the game on Survivor difficulty without using a Vita-chamber

The player has completed the game on the Hard difficulty setting

The player has taken a picture of Sander Cohen's corpse

Successfully invent at least 100 items

Used your first "emotion."

Fully research the Little Sister

Acquire all upgrades for all weapons

The player has completed the game without harvesting any Little Sisters

Purchased every slot in all four Plasmid and Tonic tracks

The player has either Harvested or Rescued every possible Little Sister

Fully upgrade five weapons

Collect or Invent 58 Tonics in the Physical, Engineering and Combat tracks

The player has broken Fontaine's mind control