The player has fully upgraded three weapons

The player has defeated Andrew Ryan

Fully research the Little Sister

The player has completed Sander Cohen's great masterpiece

The player has purchased every slot in one of the Plasmid or Tonic tracks

The player has successfully invented all possible ammo types

Hit the jackpot at a slot machine

Fully research the Houdini Splicer

The player has successfully completed 50 hacks

The player has successfully invented at least one item

The player has defeated Peach Wilkins

The player has taken at least one Research Photo of a Splicer