Fully research the Little Sister

Fully research the Houdini Splicer

The player has fully researched the Leadhead Splicer

The player has defeated Atlas

The player has become a Big Daddy

The player has broken Fontaine's mind control

The player has fully upgraded three weapons

The player has completed Sander Cohen's great masterpiece

The player has restored the forests of Arcadia

The player has purchased every slot in one of the Plasmid or Tonic tracks

The player has successfully invented at least one item

The player has defeated Peach Wilkins

The player has taken a Research Photo of the highest grade