The player has defeated Atlas

The player has either Harvested or Rescued every possible Little Sister

Purchased every slot in all four Plasmid and Tonic tracks

The player has broken Fontaine's mind control

The player has taken a picture of Sander Cohen's corpse

Ace Act 5: The Boss

Fire 5,000 paintballs

Fully upgrade four weapons

The player has defeated Andrew Ryan

The player has purchased every slot in one of the Plasmid or Tonic tracks

Fully research the Bouncer

Hit the jackpot at a slot machine