Max out all possible research

Fully research the Nitro Splicer

Perform as the guitarist

The player has purchased every slot in one of the Plasmid or Tonic tracks

The player has fully upgraded two weapons

Fully research the Little Sister

The player has fully researched the Spider Splicer

The player has successfully invented all possible ammo types

The player has restored the forests of Arcadia

The player has fully researched the Thuggish Splicer

The player has acquired at least one weapon upgrade

The player has defeated Peach Wilkins