Fully research the Rosie

The player has defeated Andrew Ryan

The player has purchased every slot in one of the Plasmid or Tonic tracks

The player has taken a picture of Sander Cohen's corpse

Fully research the Bouncer

The player has fully researched the Spider Splicer

The player has successfully invented all possible ammo types

The player has restored the forests of Arcadia

The player has successfully invented at least one item

The player has defeated Peach Wilkins

The player has shocked an enemy in the water

The player has taken at least one Research Photo of a Splicer