Collect or Invent 58 Tonics in the Physical, Engineering and Combat tracks

Fully upgrade five weapons

Fully research the Little Sister

Fully research the Nitro Splicer

The player has defeated Andrew Ryan

The player has purchased every slot in one of the Plasmid or Tonic tracks

Fully research the Houdini Splicer

Hit the jackpot at a slot machine

The player has completed Sander Cohen's great masterpiece

Collect your first flying Landscape page.

Paint a total of 20 streams of Stars.

Bring your first biped Genie to life.