Complete the game on Survivor difficulty without using a Vita-chamber

The player has completed the game on the Hard difficulty setting

The player has completed the game without harvesting any Little Sisters

Successfully invent at least 100 items

The player has either Harvested or Rescued every possible Little Sister

Acquire all upgrades for all weapons

The player has broken Fontaine's mind control

The player has entered Sander Cohen's personal quarters

Collect or Invent 58 Tonics in the Physical, Engineering and Combat tracks

Max out all possible research

Fully research the Rosie

The player has successfully invented all possible ammo types