Complete the game on Survivor difficulty without using a Vita-chamber

The player has completed the game on the Hard difficulty setting

The player has completed the game without harvesting any Little Sisters

The player has become a Big Daddy

Fully upgrade five weapons

The player has broken Fontaine's mind control

Collect or Invent 58 Tonics in the Physical, Engineering and Combat tracks

The player has entered Sander Cohen's personal quarters

Fully research the Nitro Splicer

The player has defeated Andrew Ryan

The player has completed Sander Cohen's great masterpiece

The player has fully upgraded two weapons

The player has fully researched the Spider Splicer