Complete the game on Hard difficulty without using a Vita-Chamber

The player has completed the game on the Hard difficulty setting

Charge the seventh neuron

Rescued the Little Sister in “Worlds of Hurt” using only plasmids, tonics, the wrench, and the research camera, on Medium or higher difficulty

Found all collectable Roses in “Worlds of Hurt”

Bought an amulet.

Bought a ring.

Checked the map screen.

Destroy an arrow in mid-air.

Completed the training level.

Charge the sixth neuron

Completed the mission "Return of Captain Chef".