Fully research the Little Sister

Successfully invent at least 100 items

Fully research the Nitro Splicer

The player has fully researched the Thuggish Splicer

The player has completed the game on the Hard difficulty setting

The player has completed the game without harvesting any Little Sisters

Purchased every slot in all four Plasmid and Tonic tracks

The player has either Harvested or Rescued every possible Little Sister

Fully upgrade five weapons

Collect or Invent 58 Tonics in the Physical, Engineering and Combat tracks

The player has taken a picture of Sander Cohen's corpse

The player has defeated Andrew Ryan

The player has completed Sander Cohen's great masterpiece