Purchased every slot in all four Plasmid and Tonic tracks

Fully research the Bouncer

Fully upgrade five weapons

Fully research the Nitro Splicer

Collect or Invent 58 Tonics in the Physical, Engineering and Combat tracks

The player has taken a picture of Sander Cohen's corpse

Fully research the Little Sister

The player has defeated Andrew Ryan

The player has successfully completed 50 hacks

The player has fully upgraded two weapons

The player has fully researched the Spider Splicer

The player has successfully invented at least one item

The player has purchased every slot in one of the Plasmid or Tonic tracks