The player has completed the game on the Hard difficulty setting

The player has completed the game without harvesting any Little Sisters

Find every audio diary

Collect or Invent 58 Tonics in the Physical, Engineering and Combat tracks

Acquire all upgrades for all weapons

Fully upgrade five weapons

Max out all possible research

The player has taken a picture of Sander Cohen's corpse

Fully research the Little Sister

The player has fully upgraded three weapons

Fully research the Nitro Splicer

The player has purchased every slot in one of the Plasmid or Tonic tracks

The player has fully upgraded two weapons