The player has completed the game on the Hard difficulty setting

Find every audio diary

Successfully invent at least 100 items

The player has completed the game without harvesting any Little Sisters

Purchased every slot in all four Plasmid and Tonic tracks

Max out all possible research

Collect or Invent 58 Tonics in the Physical, Engineering and Combat tracks

Fully research the Little Sister

The player has broken Fontaine's mind control

Fully research the Nitro Splicer

Take at least one photo in every research group

The player has fully researched the Thuggish Splicer