The player has entered Sander Cohen's personal quarters

Fully upgrade four weapons

Fully research the Little Sister

Fully research the Nitro Splicer

The player has fully upgraded three weapons

The player has completed Sander Cohen's great masterpiece

Take at least one photo in every research group

The player has fully researched the Spider Splicer

The player has successfully invented all possible ammo types

The player has purchased every slot in one of the Plasmid or Tonic tracks

Fully research the Houdini Splicer

The player has successfully invented at least one item

The player has defeated Peach Wilkins