Max out all possible research

Complete the game on Hard difficulty without using a Vita-Chamber

The player has defeated Atlas

The player has become a Big Daddy

The player has either Harvested or Rescued every possible Little Sister

Purchased every slot in all four Plasmid and Tonic tracks

Successfully invent at least 100 items

Fully research the Rosie

The player has entered Sander Cohen's personal quarters

The player has taken a picture of Sander Cohen's corpse

The player has defeated Andrew Ryan

The player has completed Sander Cohen's great masterpiece

Fully research the Houdini Splicer