The player has completed the game without harvesting any Little Sisters

The player has successfully hacked a turret

Fully research the Little Sister

Fully upgrade five weapons

Purchased every slot in all four Plasmid and Tonic tracks

Collect or Invent 58 Tonics in the Physical, Engineering and Combat tracks

The player has purchased every slot in one of the Plasmid or Tonic tracks

Fully upgrade four weapons

The player has fully upgraded three weapons

The player has fully upgraded two weapons

The player has fully researched the Spider Splicer

The player has fully researched the Thuggish Splicer

The player has fully researched the Leadhead Splicer