The player has become a Big Daddy

Acquire all upgrades for all weapons

Fully upgrade five weapons

Collect or Invent 58 Tonics in the Physical, Engineering and Combat tracks

The player has entered Sander Cohen's personal quarters

Fully upgrade four weapons

Fully research the Little Sister

The player has defeated Andrew Ryan

The player has purchased every slot in one of the Plasmid or Tonic tracks

The player has fully researched the Spider Splicer

Hit the jackpot at a slot machine

The player has restored the forests of Arcadia