The player has taken a picture of Sander Cohen's corpse

Purchased every slot in all four Plasmid and Tonic tracks

The player has defeated Andrew Ryan

The player has completed Sander Cohen's great masterpiece

The player has fully researched the Spider Splicer

The player has purchased every slot in one of the Plasmid or Tonic tracks

The player has fully upgraded two weapons

Acquire the BFG

Earn the Mastery for a weapon mod

Use explosive barrels to kill 100 enemies

The player has successfully completed 50 hacks

The player has fully researched the Thuggish Splicer