The player has either Harvested or Rescued every possible Little Sister

Acquire all upgrades for all weapons

Collect or Invent 58 Tonics in the Physical, Engineering and Combat tracks

The player has broken Fontaine's mind control

The player has taken a picture of Sander Cohen's corpse

Fully upgrade four weapons

The player has defeated Andrew Ryan

The player has purchased every slot in one of the Plasmid or Tonic tracks

Fully research the Little Sister

The player has fully upgraded two weapons

The player has successfully invented all possible ammo types

Hit the jackpot at a slot machine