Enter the Prison.

Enter the Tanker 2 level.

Take down another Queen in Emerald Isle.

Defeat the Hive Queen at the Strip Mine.

Eliminate three bugs at once with a bomb.

Enter the Construction Site 2 level.

Enter the Fish Cannery level.

Obtain the Flame Thrower upgrade from Taryn.

Enter the Tanker level.

Defeat three Queens in Breezy Valley.

Defeat a bug with a flamethrower.

Defeat the Hive Queen under the Transit System.

Enter the Transit System level.