The player has defeated Atlas

Collect or Invent 58 Tonics in the Physical, Engineering and Combat tracks

The player has either Harvested or Rescued every possible Little Sister

The player has purchased every slot in one of the Plasmid or Tonic tracks

Fully upgrade four weapons

The player has defeated Andrew Ryan

The player has fully upgraded three weapons

The player has completed Sander Cohen's great masterpiece

The player has fully researched the Spider Splicer

The player has restored the forests of Arcadia

The player has successfully invented at least one item

The player has acquired at least one weapon upgrade

The player has taken a Research Photo of the highest grade