Defeated 3 Big Daddies without dying during the fight.

Kill a Lancer Big Daddy

The player has defeated Atlas

The player has either Harvested or Rescued every possible Little Sister

The player has purchased every slot in one of the Plasmid or Tonic tracks

Collect or Invent 58 Tonics in the Physical, Engineering and Combat tracks

Fully research the Nitro Splicer

The player has defeated Andrew Ryan

The player has successfully completed 50 hacks

The player has taken a picture of Sander Cohen's corpse

The player has fully upgraded one weapon

The player has restored the forests of Arcadia

The player has fully researched the Leadhead Splicer