The player has completed the game without harvesting any Little Sisters

Successfully invent at least 100 items

The player has successfully hacked a turret

The player has either Harvested or Rescued every possible Little Sister

The player has fully upgraded three weapons

The player has defeated Andrew Ryan

The player has completed Sander Cohen's great masterpiece

The player has purchased every slot in one of the Plasmid or Tonic tracks

The player has successfully invented all possible ammo types

The player has successfully invented at least one item

The player has defeated Peach Wilkins

The player has taken a Research Photo of the highest grade

The player has successfully hacked a vending machine