The player has purchased every slot in one of the Plasmid or Tonic tracks

The player has successfully hacked a turret

The player has defeated Peach Wilkins

The player has taken at least one Research Photo of a Splicer

The player has defeated the crazed Dr. Steinman

The player has successfully hacked a vending machine

The player has performed at least one successful hack

The player has shocked an enemy in the water