Purchased every slot in all four Plasmid and Tonic tracks

The player has become a Big Daddy

The player has broken Fontaine's mind control

The player has successfully invented all possible ammo types

The player has purchased every slot in one of the Plasmid or Tonic tracks

The player has successfully invented at least one item

The player has defeated Peach Wilkins

The player has taken a Research Photo of the highest grade

The player has defeated the crazed Dr. Steinman

The player has successfully hacked a turret

The player has performed at least one successful hack

The player has shocked an enemy in the water