Complete DNA Sequence 14.

Complete Present - Stadium.

Complete one challenge and receive at least a Bronze medal.

Defeat Iguana

Perform a Web-Rush punch

Complete Sequence 7.

Recruit any of the Artisans and see them settled on the Homestead.

Complete Present - Skyscraper.

Be invited to join a Club.

Complete Sequence 3.

Re-Enter the Animus.

Complete your first assassination mission for Lorenzo Il Magnifico.

Discover the Assassin's Tomb inside Santa Maria del Fiore (The Duomo).