Adopted a new Little Sister for the first time.

Defended yourself against Lamb's assault in the train station.

Found your way back into the ruins of Rapture.

Submit Ocelot

Shoot a Kerotan for the first time

CQC Slit an enemy's throat

Capture any animal alive

Beat the Tanker and Plant chapters on any difficulty

Defeat Metal Gear RAY

Acquire the digital camera

Defeat Vamp

Destroy the AV-88 Harrier II

Learn the location of the president