The player has entered Sander Cohen's personal quarters

Fully research the Houdini Splicer

The player has completed the game on the Hard difficulty setting

The player has defeated Atlas

Fully research the Rosie

Purchased every slot in all four Plasmid and Tonic tracks

Fully research the Nitro Splicer

Take at least one photo in every research group

The player has fully upgraded two weapons

Hit the jackpot at a slot machine

The player has successfully hacked a security camera

The player has purchased every slot in one of the Plasmid or Tonic tracks

The player has fully upgraded one weapon