Fully research the Houdini Splicer

Fully research the Nitro Splicer

Rescued the Little Sister in “A Shocking Turn of Events”

The player has completed the game without harvesting any Little Sisters

Purchased every slot in all four Plasmid and Tonic tracks

The player has fully upgraded one weapon

The player has purchased every slot in one of the Plasmid or Tonic tracks

The player has completed Sander Cohen's great masterpiece

The player has successfully invented at least one item

The player has acquired at least one weapon upgrade

The player has successfully hacked a turret

The player has taken at least one Research Photo of a Splicer

The player has defeated the crazed Dr. Steinman