The player has purchased every slot in one of the Plasmid or Tonic tracks

Fully research the Houdini Splicer

The player has fully researched the Thuggish Splicer

The player has acquired at least one weapon upgrade

The player has defeated Peach Wilkins

The player has taken at least one Research Photo of a Splicer

The player has purchased one slot in any Plasmid or Tonic track

The player has successfully hacked a security camera

The player has successfully hacked a turret

The player has successfully hacked a security bot

The player has successfully completed the Welcome To Rapture Level

Rescued the Little Sister in “Worlds of Hurt” using only plasmids, tonics, the wrench, and the research camera, on Medium or higher difficulty