The player has completed the game without harvesting any Little Sisters

Collect or Invent 58 Tonics in the Physical, Engineering and Combat tracks

Max out all possible research

The player has become a Big Daddy

The player has either Harvested or Rescued every possible Little Sister

Fully research the Rosie

The player has purchased every slot in one of the Plasmid or Tonic tracks

Fully research the Nitro Splicer

The player has taken a picture of Sander Cohen's corpse

Fully upgrade four weapons

Fully research the Bouncer

The player has completed Sander Cohen's great masterpiece

Take at least one photo in every research group