The player has defeated Atlas

Fully research the Bouncer

The player has either Harvested or Rescued every possible Little Sister

The player has become a Big Daddy

Fully upgrade five weapons

Fully research the Little Sister

Fully research the Nitro Splicer

The player has purchased every slot in one of the Plasmid or Tonic tracks

The player has defeated Andrew Ryan

Collect or Invent 58 Tonics in the Physical, Engineering and Combat tracks

Fully research the Houdini Splicer

The player has completed Sander Cohen's great masterpiece

Hit the jackpot at a slot machine