The player has completed the game without harvesting any Little Sisters

The player has become a Big Daddy

The player has either Harvested or Rescued every possible Little Sister

Fully upgrade five weapons

The player has successfully invented all possible ammo types

The player has entered Sander Cohen's personal quarters

Collect or Invent 58 Tonics in the Physical, Engineering and Combat tracks

The player has defeated Andrew Ryan

Fully research the Bouncer

Purchased every slot in all four Plasmid and Tonic tracks

The player has completed Sander Cohen's great masterpiece

The player has fully upgraded two weapons