Clear second level without taking any damage

Banish 10 threats.

Catch all known species of fish.

Find a use for the relics.

Use Atrophy on a fishing spot a long way away.

Solve all fish shrine puzzles.

Use Mixed Bait to attract 3 different species to a single spot.

Spot a fish of each category through your Spyglass.

Give up the Pocket Watch.

Research all pots.

Have a combined fishing speed of 200%.

Traverse a long distance using Manifest.