Fully research the Little Sister

The player has fully upgraded three weapons

The player has defeated Andrew Ryan

The player has fully researched the Spider Splicer

The player has purchased every slot in one of the Plasmid or Tonic tracks

The player has completed Sander Cohen's great masterpiece

Hit the jackpot at a slot machine

The player has restored the forests of Arcadia

The player has fully researched the Thuggish Splicer

The player has successfully invented at least one item

The player has defeated Peach Wilkins

The player has taken at least one Research Photo of a Splicer

The player has defeated the crazed Dr. Steinman