The player has either Harvested or Rescued every possible Little Sister

The player has become a Big Daddy

Fully upgrade five weapons

The player has purchased every slot in one of the Plasmid or Tonic tracks

Fully research the Nitro Splicer

The player has taken a picture of Sander Cohen's corpse

The player has defeated Andrew Ryan

The player has fully upgraded two weapons

The player has successfully invented all possible ammo types

The player has fully researched the Thuggish Splicer

The player has restored the forests of Arcadia

The player has fully upgraded one weapon