Complete the game on Hard difficulty without using a Vita-Chamber

The player has completed the game on the Hard difficulty setting

Clear all the maps in W3.

Find every audio diary

The player has completed the game without harvesting any Little Sisters

Collect or Invent 58 Tonics in the Physical, Engineering and Combat tracks

Max out all possible research

The player has either Harvested or Rescued every possible Little Sister

Fully upgrade five weapons

The player has broken Fontaine's mind control

The player has entered Sander Cohen's personal quarters

The player has defeated Andrew Ryan

Perform a 3-part Hyper Combo (Multiplayer)