Purchased every slot in all four Plasmid and Tonic tracks

Fully research the Bouncer

The player has completed Sander Cohen's great masterpiece

The player has fully upgraded two weapons

Hit the jackpot at a slot machine

The player has purchased every slot in one of the Plasmid or Tonic tracks

Kill 30 enemies by shooting a fire arrow at oil jars.

The player has fully upgraded one weapon

The player has fully researched the Thuggish Splicer

The player has acquired at least one weapon upgrade

Fully research the Nitro Splicer

Complete Side Quest "Seven Farmers".

Complete the last Main Quest.