Rescued the Little Sister in “The ‘I’ in Team”

The player has completed the game on the Hard difficulty setting

Complete the game on Survivor difficulty without using a Vita-chamber

Complete the game on Survivor difficulty

The player has defeated Atlas

Collect or Invent 58 Tonics in the Physical, Engineering and Combat tracks

Successfully invent at least 100 items

The player has either Harvested or Rescued every possible Little Sister

Fully upgrade five weapons

The player has entered Sander Cohen's personal quarters

Fully upgrade four weapons

The player has defeated Andrew Ryan

Purchased every slot in all four Plasmid and Tonic tracks